The Biocatalytic Degradation of Organic Dyes Using Laccase Immobilized Magnetic Nanoparticles

Free laccase has limitations for its use in industrial applications that require immobilization on proper support, to improve catalytic activity. Herein, the nanoparticles of magnetic iron oxide (Fe3O4) and copper ferrite (CuFe2O4) were successfully used as support free laccase, using glutaraldehyde a cross-linker. The conditions surface optimized reach maximum activity immobilized enzyme. synthesized nanoparticle-immobilized characterized different techniques, including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM), vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA). CuFe2O4 nanoparticles, enhanced compared Fe3O4 appeared during study pH, temperature, storage stability laccase. showed superior wide pH range, temperature period, up 20 days at 4.0 °C, when Additionally, nanobiocatalysts examined biodegradation anionic dye Direct Red 23 (DR23). HPLC was confirm degradation. reusability laccases DR23 investigated six successive cycles, with decolorization efficiency over 70.0%, which indicated good excellent stability.